COMPARISON BETWEEN RELATIVE AND ABSOLUTE QUANTITATIVE REAL-TIME PCR APPLIED TO SINGLE-CELL ANALYSES: TRANSCRIPTIONAL LEVELS IN A KEY NEURON FOR LONG-TERM MEMORY IN THE POND SNAIL.

Quantitative real-time PCR (qPCR) is a powerful method for measuring nucleic acid levels and quantifying mRNA levels, even in single cells.In the present study, we compared the results of single-cell qPCR obtained by different quantification methods (relative and absolute) and different reverse transcription methods.In the experiments, we focused o

Abstract American Indian and Alaska Native (AI/AN) individuals are more likely to die with COVID-19 than other groups, but there is limited empirical evidence to explain the cause of this inequity.The objective of this study was to determine whether medical comorbidities, area socioeconomic deprivation, or access to treatment can explain the greate

ABSTRACT The enterococci are Gram-positive lactic acid bacteria that inhabit the gastrointestinal tracts of diverse hosts.However, Enterococcus faecium and E.faecalis have emerged as leading causes of multidrug-resistant hospital-acquired infections.The mechanism by which a well-adapted commensal evolved into a hospital pathogen is poorly understoo